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A Parametric Study on the Continuous Production of Lactic Acid by Immobilized Lactobacillus acidophilus

Author : Monteiro, Maida Sombilla
Major Adviser : Chay, Pham Binh
Committee Members : Movillon, Jovita A.; Acda, Reynaldo I.
Year : 1996
Month : April
Type : Thesis
Degree: BS
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Abstract

Continuous production of lactic acid by immobilized Lactobacillus acidophilus was studied. The cells were immobilized in calcium-alginate solution and a continuous stirred tank reactor was used.

The effects of dilution rate (0.008, 0.010 and 0.030 hr⁻¹), cell loading (500, 600 and 700 mL de Mans-Rogosan Sharfor seed medium/150 mL gel) and pH (5.0, 5.5 and 6.0) were determined. Fermentation was carried out for 192 hours for each treatment.

Highest specific growth rate, 0.13 hr⁻¹ and highest specific production rate, 0.67 g*L⁻¹*hr⁻¹ was attained at D = 0.030 hr⁻¹, 700 mL fermentation medium loaded to the gel and at a maintained pH 6.0. Highest specific consumption rate was at a maintained pH 5.5. The process proved to be useful for prolonged production of lactic acid.

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