A Study on Micro-gels Beads Formation for Microbial Immobilization
Author : Soriano, Ramon Leo Sison
Major Adviser : Chay, Pham Binh
Committee Members :
Year : 1990
Month : March
Type : Thesis
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An immobilization system was fabricated based on the principle of atomization by the rotating disk.
The effects of the speed of the rotating disk, the feed rate, and the concentration of the Na-alginate solution on the diameter, volume, and shape of the micro-gel beads produced were evaluated.
A test was also conducted on the micro-gel beads by immobilizing Corynebacterium glutamicum to evaluate the glucose consumption and lysine production by the fermentation process.
Rotating the disk at a speed of 1000 rpm, Na-alginate solution concentration of 2.0%, and a Na-alginate solution feed rate of 1.5 ml/min produced the best micro-gel beads in terms of diameter (0.0992 cm) and uniformity with standard deviation of 0.00376.
The production of lysine after three days of fermentation showed that the cells immobilized in the micro-gel beads were not limited by the process used.
The immobilization system requires further study to produce smaller gel beads and determine the best concentration of CaCl2 that can be used. The kinetic parameters and other applications of the micro-gel beads produced should also be studied.
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