Optimization of Denaturant Concentration, Crosslinker Concentration, and Pressing Time for Bioadhesive Production From Chlorella vulgaris Protein Extract
Author : Lopez, Angelica Marinay
Major Adviser : Guerrero, Gino Apollo M.
Major Co-Adviser : Ventura, Jey-R S.
Committee Members : Alcantara, Jerico Z.; Detras, Monet Concepcion M.
Year : 2019
Month : July
Type : Thesis
Degree: BS
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Abstract
Recent studies revealed that microalgae can be a good source of protein that can be used for bioadhesive production. Protein extracted from Chlorella vulgaris was used in this study to produce the wood adhesive. Studies made before the experiment were consulted for the selection of significant factors. The optimization of the production process based on the effects of denaturant concentration, crosslinker concentration, and pressing time was done to compare the shear strength from commercial wood adhesives. It was found that there were two sets of optimum conditions for bioadhesive production: (1) 199.10 mM NaOH, 2.81 % (v/v) glyoxal, and 581 seconds pressing time; and (2) 56.43 mM NaOH, 7.98 % (v/v) glyoxal, and 556 seconds pressing time. The two optimum regions were caused by the interaction of denaturant concentration and crosslinker concentration. In general, a higher denaturant concentration will result to a higher shear strength but is only limited to a certain range depending on the isoelectric point of protein. Same goes with the crosslinker where higher concentrations lead to better bonding strength. There is no significant difference between the shear strength yield between the adhesives produced from the two sets of optimum conditions. Between the two sets of optimum conditions, the first set of values (199.10 mM NaOH, 2.81% (v/v) glyoxal, 581 seconds pressing time) might be a better option if the cost of materials will be considered. The maximum shear strength from the study was found to be 3.7130 MPa which was produced using the following conditions: 56.43 mM NaOH concentration, 7.98 % (v/v) glyoxal concentration, and 556 seconds pressing time. This study was able to successfully produce a wood adhesive that has a shear strength comparable with commonly used commercial adhesives and could be further studied for possible uses other than being adhesives for woods.
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