Parametric and Optimization Study on the Enzymatic Extraction of Pectin from Mango Peels
Author : Bondoc, Yves David
Major Adviser : Movillon, Jovita A.
Major Co-Adviser : Sapin, Arsenia B.
Committee Members : Alfafara, Catalino G.; Arocena, Rhebner E.
Year : 2016
Month : June
Type : Thesis
Degree: BS
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Abstract
Utilization of by-product such as mango peels in the production of valuable product like pectin is one of the emerging concerns nowadays. The enzymatic way of producing pectin is a promising step in lessening environmental impact such as pollution and toxicity due to chemicals. For this study, the enzyme used to liberate the pectin was Cellulase (Sigma C-9748). The effect of the three factors (solids loading, enzyme loading and reaction time) on the responses (pectin yield and galacturonic acid yield) were determined significant through the two-level factorial design. Response-surface methodology (Box-Behnken) showed statistically significant quadratic model. Based on the numerical optimization, the predicted pectin yield (20.01 % w/w) and galacturonic acid yield (147.711 mg/g) shall be obtained at 2 % (w/v) solids loading, 3.598 % (w/w) and 11.562 (hour) reaction time. The experimental verification of optimum conditions (2 % w/v solids loading, 3.599 % w/w enzyme loading and 11.561-hour reaction time) showed a pectin yield of 19.59 % (w/v) and a galacturonic acid yield of 146.307 mg/g. The predicted and actual values were compared and the percent difference for pectin yield was 2.10 % and 0.95 % for galacturonic acid.
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