Parametric Study of Microwave-Assisted Extraction of Runo (Miscanthus sinensis Andersson) Dye and its Application to Paper & Cotton Fabric
Author : Pinzon, Ivan Anthony Protacio
Major Adviser : Ramon A. Razal
Major Co-Adviser : Carpio, Rowena B.
Committee Members : Mendoza, Rosalie C; Eusebio, Ramon Christian P.
Year : 2019
Month : June
Type : Thesis
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The study was designed to devise a high-yielding, microwave-assisted extraction of the colored substance from the core tissue in the stem of runo (Miscanthus sinensis Andersson). Soxhlet extraction of ground M. sinensis samples gave yield ranging from 1.04% with dichloromethane as solvent to 11.91% from 1:1 ethanol-water mixture solvent extraction. Folin-Ciocalteau tests showed that the extracts were primarily flavonoid compounds, accounting for 66.0506 ppm of the total 1:1 ethanol-water extractable material. Application trials of the ethanol, ethyl acetate, and ethanol-water extracts followed by wash and light fastness tests showed poor retention on both paper and cotton fabric for the extracts using the three solvents, suggesting the need for a mordant. Subsequent trials with two different mordants showed that aluminum acetate greatly improved binding of the ethanol-water extracted Runo dye onto the cotton fabric. A two-level, full factorial model extraction procedure to determine the effects of extraction time (15 s – 90 s), solvent volume (50 mL – 150 mL), and microwave power level (90 W – 540 W) was done. All three factors had a significant effect on the dye extraction yield, along with the interactions between duration-power level and volume-power level. Results suggest that microwave extraction can potentially produce dye extracts from Runo with a faster throughput than simple soaking and Soxhlet extraction.
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