Parametric Study on the Ultrasound-Assisted Extraction of Phenolic Compounds from Indian Goosegrass (Eleusine indica (L.) Gaertn.) Using Aqueous Ethanol as Solvent
Author : Amoranto, Ma. Jonah Girl Herradura
Major Adviser : Arocena, Rhebner E.
Committee Members : Capunitan, Jewel A.; Migo-Sumagang, Maria Victoria P.
Year : 2019
Month : June
Type : Thesis
Degree: BS
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Abstract
Weeds have become a nuisance to farmers by increasing the production cost while decreasing the crop yield. However, these weeds contain bioactive compounds which act as antioxidants and are valuable in the pharmaceutical and food industries. This research focused on the parametric study on the ultrasound-assisted extraction of phenolic compounds from indian goosegrass (Eleusine indica (L). Gaertn.) using aqueous ethanol and FCR method for analysis. It was found out that the fresh indian goosegrass contains 67.51 %(w/w) moisture and the bone dry weight is 32.49 %(w/w). By using multiple extraction, the obtained maximum TPC was 517.76 ± 9.80 mg GAE/100 g dry feedstock. There were four parameters considered in this study: A: ultrasonication (w/ and w/o), B: temperature (20 °C and 50 °C), C: extraction time (10 mins and 40 mins), and D: solvent concentration (25%v/v and 75%v/v). Only B, C, and interaction between A and D were found to be significant based on partial fractional factorial 2k-1 analysis at 95% level of confidence using Design Expert 11 Trial Version. The highest TPC measured was 512.37 mg GAE/100 g dry feedstock which happened at the centerpoint (35 °C, 25 mins, 50% ethanol) when there is ultrasonication. It gave 98.96% recovery while the lowest was 39.01 mg GAE/100 g dried feedstock occurring at 50 °C, 10 mins, and 25% ethanol when there is ultrsonication, which gave a percent recovery of 7.53%, The high recovery percentage dictates significance of the ultrasonication method. The observed highest TPC at the centerpoint could be a basis for optimization studies.
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