Efficacy Of Pleurotus sajor-caju Biotech 3031 Mycelium Production Using Submerged Culture Process

Author : Sanoy, Ma. Rebecca M.
Major Adviser : Chay, Pham Binh
Committee Members : Movillon, Jovita L.; Alcanzare, Edilberto A.
Year : 1994
Month : April
Type : Thesis
Degree: BS
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Abstract

The efficacy of producing mycelia of Pleurotus sajor-caju BIOTECH 3031 submerged culture process was determined. The effects of pH, aeration rate and temperature were tested and evaluated. Results of the analysis of variance (ANOVA) using between-group analysis showed that the yield is significantly affected by pH and aeration rate levels. Temperature also produced effects but to a lesser extend compared with the pH and aeration rate.

Duncan’s Multiple Range Test (DMRT) together with between-group comparisons showed that Pleurotus sajor-caju BIOTECH 3031 subjected to pH, aeration rate and temperature of 0.05 v/vm, 5.0 and 25°C, respectively, gave the highest yield, was significantly different from the rest of the treatments tested. A regression equation was developed to describe the yield behavior as affected by pH, aeration rate and temperature.

The specific growth rate of the organism was found to be equal to 0.0832/hr, at the highest yield of the organism obtained experimentally.

It is recommended to improve the apparatus to maintain constancy of controls of the desired conditions to be tested. Further tests are required considering a wider range of the three process variables in order to attain an optimum condition and a good predicting equation. It is also necessary to test other process variables related to the growth of the organism which includes dissolved oxygen.

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