Growth Kinetics and Solvents (Acetone, Butanol and Ethanol) Production of Clostridium acetobutylicum Fe 843 Strain Using Continuous Fermentation Process

Author : Mora, Maribel Garcia
Major Adviser : Calibo, Ronnie L.
Committee Members : Arquiza, Apollo C.; Movillon, Jovita L.
Year : 1994
Month : April
Type : Thesis
Degree: BS
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Abstract

The growth kinetics and solvents (acetone, butanol and ethanol) production of Clostridium acetobutylicum FE 843 strain was determined using continuous culture technique with molasses having an initial 65 g/L glucose concentration as substrate. The overall results showed that biomass production, products formation and substrate consumption strongly decreased with increasing dilution rate. The maximum steady state concentrations substrate, biomass and products with concentrations of 41.33 g/L, 22.12 g/L and 19.24 g/L, respectively, were obtained at the lowest investigated dilution rate of 0.05/hour while at the maximum dilution rate of 0.16/hour unstable culture behaviors were observed. A simple method of writing stoichiometric equations representing the given sets of data and the kinetic ratio of solvents produced were formulated and established. The final ratio of acetone, butanol and ethanol (ABE) obtained were 6:8:1 (Trial 1), 2:4:1 (Trial 2) and 6:7:1 (Trial 3) for D = 0.05/hour; and 3:5:1 (Trial 1), 8:13:1 (Trial 2) and 5:9:1 (Trial 3) for D = 0.11/hour.

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