Parametric Study on the Ethanol Fermentation of Sweet Sorghum [Sorghum bicolor (L.) Moench ‘SPV 422’] Syrup Using Different Yeast Strains Under Sterilized and Unsterilized Conditions
Author : Relleve, Karla Restubog
Major Adviser : Demafelis, Rex B.
Committee Members : Borines, Myra G.; Movillon, Jovita L.
Year : 2015
Month : July
Type : Thesis
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Ethanol fermentation was conducted using sweet sorghum (SPV 422) syrup as substrate to evaluate the effects of yeast strain, pH, inoculum loading and sterilization on the ethanol concentration and sugar consumption. Two local BIOTECH yeast strains were used namely: Saccharomyces cerevisiae 2011 and Saccharomyces cerevisiae 2055. Among the parameters tested, pH, inoculum loading and sterilization were found to have a significant effect on the final ethanol concentration. Yeast strain and sterilization had a significant effect on reducing sugar consumption, while inoculum loading had a significant effect on total sugar consumption. Saccharomyces cerevisiae 2011 was selected over Saccharomyces cerevisiae 2055 since higher ethanol concentration and sugar consumption were achieved using this strain. The highest ethanol concentration obtained was 8.73% with 94.69% reducing sugar consumption. A fermentation profile using the optimum conditions (pH 4.5, 10% inoculum loading and with sterilization) was constructed and a fermentation efficiency of 84.66% was achieved.
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